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Anti-phospho-Ser/Thr ATM/ATR Substrate Antibody (A309536)

Western Blot - Anti-phospho-Ser/Thr ATM/ATR Substrate Antibody (A309536) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to phospho-Ser/Thr ATM/ATR Substrate for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-phospho-Ser/Thr ATM/ATR Substrate Antibody
Description
Rabbit polyclonal antibody to phospho-Ser/Thr ATM/ATR Substrate.
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence containing phosphorylated S & T.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
38 - 68 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-phospho-Ser/Thr ATM/ATR Substrate Antibody (A309536) - Antibodies.comEnlarge Image

Western Blot - Anti-phospho-Ser/Thr ATM/ATR Substrate Antibody (A309536)

Western blot analysis of extracts of various cell lines, using Anti-phospho-Ser/Thr ATM/ATR Substrate Antibody (A309536) at 1:1,000 dilution. Jurkat and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. C6 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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