Primary Antibodies
Secondary Antibodies
Proteins & Peptides
ELISA Kits
About Us
Contact Us
Antibodies.com Logo Antibodies.com Logo
Sign In/Register
0
ISO 9001:2015 Certified
Live Customer Support
4.5/5 on Trustpilot
100% Quality Guarantee

Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A93574)

Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.com Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.com Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.com Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.com Immunohistochemistry - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.com Immunohistochemistry - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.com Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.com +1
$200
Product Datasheet

Rabbit polyclonal antibody to PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) for WB, IF and ELISA.

100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody
Description
Rabbit polyclonal antibody to PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199).
Specificity
This antibody detects endogenous levels of PI3-kinase p85-alpha/gamma only when phosphorylated at Tyr467/199.
Applications
WB, IF, ELISA
Dilutions
WB: 1:500~1:1000, IF: 1:100~1:500, ELISA: 1:40000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199 (amino acids 436-485).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
83kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (8)

Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.comEnlarge Image

Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A93574)

Western blot analysis of lysates from COS7 cells treated with H2O2 100uM 30' using Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A93574)

Immunofluorescence analysis of NIH/3T3 cells using Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.comEnlarge Image

Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A93574)

Western blot analysis of various cells using Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody at 1:1,000 (4°C overnight). Goat Anti-Rabbit IgG (IRDye 800) was used as a secondary at 1:5,000 (25°C, 1 hour).
Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A1224) - Antibodies.comEnlarge Image

Western Blot - Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A93574)

Western blot analysis of COS7 cells using Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody.
Publishing research using Anti-PI 3 Kinase p85 alpha + Gamma (phospho Tyr467 + Tyr199) Antibody (A93574)? Please let us know so that we can list the citation on this page.