Rabbit polyclonal antibody to PI3-kinase p85- alpha (Phospho-Tyr607)
Specificity
The antibody detects endogenous level of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607.
Applications
WB
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3-kinase p85-alpha.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Rat liver tissue using PI3-kinase p85- alpha (Phospho-Tyr607) Antibody #12057.The lane on the right is treated with the antigen-specific peptide.
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