The antibody detects endogenous level of total PI3 Kinase p85 / p55 only when phosphorylated at tyrosine 467 / 199.
Applications
WB, ICC/IF
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85 / p55.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Predicted MW
55, 85kd
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from 293 cells untreated or treated with sorbitol using PI3 Kinase p85/p55 (phospho-Tyr467/199)Antibody #11508.
Publishing research using Anti-PI3 Kinase p85 / p55 (phospho-Tyr467 / 199)Antibody Antibody (A50616)? Please let us know so that we can list the citation on this page.
