The antibody detects endogenous level of total PIAS1 antibody.
Applications
WB, IF
Dilutions
Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human PIAS1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were purified by affinity purification using immunogen.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, E3 SUMO-protein transferase PIAS1, GBP, Gu-binding protein, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using PIAS1 antibody. Green£ºGFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
![Western Blot - Anti-PIAS1 Antibody [ARC1116] (A305639) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305639_1.jpg?profile=product_alternative)