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Anti-PIWIL4/PIWI Antibody (A87778)

Western Blot - Anti-PIWIL4/PIWI Antibody (A87778) - Antibodies.com Immunohistochemistry - Anti-PIWIL4/PIWI Antibody (A87778) - Antibodies.com Immunohistochemistry - Anti-PIWIL4/PIWI Antibody (A87778) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to PIWIL4/PIWI for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-PIWIL4/PIWI Antibody
Description
Rabbit polyclonal antibody to PIWIL4/PIWI.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIWIL4 (NP_689644.2).
Sequence
SADVSYKVLRNETVLEFMTALCQRTGLSCFTQTCEKQLIGLIVLTRYNNRTYSIDDIDWSVKPTHTFQKRDGTEITYVDYYKQQYDITVSDLNQPMLVSLLKKKRNDNSEAQLAHLIPELCFLTGLTDQATSDFQLMKAVAEKTRLSPSGRQQRLARLVDNIQRNTNARFELETWGLHFGSQISLTGRIVPSEKILMQDHI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
97 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-PIWIL4/PIWI Antibody (A87778) - Antibodies.comEnlarge Image

Western Blot - Anti-PIWIL4/PIWI Antibody (A87778)

Western blot analysis of extracts of various cell lines, using Anti-PIWIL4/PIWI Antibody (A87778) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 10s.
Immunohistochemistry - Anti-PIWIL4/PIWI Antibody (A87778) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PIWIL4/PIWI Antibody (A87778)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-PIWIL4/PIWI Antibody (A87778) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PIWIL4/PIWI Antibody (A87778) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PIWIL4/PIWI Antibody (A87778)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-PIWIL4/PIWI Antibody (A87778) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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