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Anti-PKA R2/PKR2 Antibody (A13489)

Western Blot - Anti-PKA R2/PKR2 Antibody (A13489) - Antibodies.com Western Blot - Anti-PKA R2/PKR2 Antibody (A13489) - Antibodies.com Immunofluorescence - Anti-PKA R2/PKR2 Antibody (A13489) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to PKA R2/PKR2 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-PKA R2/PKR2 Antibody
Description
Rabbit polyclonal antibody to PKA R2/PKR2.
Applications
WB, ICC/IF
Dilutions
WB: 1:1,000-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PKA RIIa (PRKAR2A)/PKR2 (NP_004148.1).
Sequence
MSHIQIPPGLTELLQGYTVEVLRQQPPDLVEFAVEYFTRLREARAPASVLPAATPRQSLGHPPPEPGPDRVADAKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQGEKADSFYIIESGEVSILIRSRTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
46 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-PKA R2/PKR2 Antibody (A13489) - Antibodies.comEnlarge Image

Western Blot - Anti-PKA R2/PKR2 Antibody (A13489)

Western blot analysis of extracts from normal (control) and PKA RIIalpha (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using Anti-PKA R2/PKR2 Antibody (A13489) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Western Blot - Anti-PKA R2/PKR2 Antibody (A13489) - Antibodies.comEnlarge Image

Western Blot - Anti-PKA R2/PKR2 Antibody (A13489)

Western blot analysis of extracts of various cell lines, using Anti-PKA R2/PKR2 Antibody (A13489) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence - Anti-PKA R2/PKR2 Antibody (A13489) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PKA R2/PKR2 Antibody (A13489)

Immunofluorescence analysis of L929 cells using Anti-PKA R2/PKR2 Antibody (A13489) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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