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Anti-PKC-pan (phospho Thr497) Antibody (A94904)

Western Blot - Anti-PKC-pan (phospho Thr497) Antibody (A0803) - Antibodies.com Immunohistochemistry - Anti-PKC-pan (phospho Thr497) Antibody (A0803) - Antibodies.com Immunofluorescence - Anti-PKC-pan (phospho Thr497) Antibody (A0803) - Antibodies.com Western Blot - Anti-PKC-pan (phospho Thr497) Antibody (A0803) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to PKC-pan (phospho Thr497) for WB, IHC, IF and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-PKC-pan (phospho Thr497) Antibody
Description
Rabbit polyclonal antibody to PKC-pan (phospho Thr497).
Specificity
This antibody detects endogenous levels of PKC-pan only when phosphorylated at Thr497.
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:100-1:500, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human PKC-pan around the phosphorylation site of Thr497 (amino acids 623-672).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
76kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-PKC-pan (phospho Thr497) Antibody (A0803) - Antibodies.comEnlarge Image

Western Blot - Anti-PKC-pan (phospho Thr497) Antibody (A94904)

Western blot analysis of lysates from NIH/3T3 cells treated with PMA 250ng/ml 15' using Anti-PKC-pan (phospho Thr497) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemistry - Anti-PKC-pan (phospho Thr497) Antibody (A0803) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PKC-pan (phospho Thr497) Antibody (A94904)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-PKC-pan (phospho Thr497) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence - Anti-PKC-pan (phospho Thr497) Antibody (A0803) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PKC-pan (phospho Thr497) Antibody (A94904)

Immunofluorescence analysis of HepG2 cells using Anti-PKC-pan (phospho Thr497) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Western Blot - Anti-PKC-pan (phospho Thr497) Antibody (A0803) - Antibodies.comEnlarge Image

Western Blot - Anti-PKC-pan (phospho Thr497) Antibody (A94904)

Western blot analysis of 293 cells using Anti-PKC-pan (phospho Thr497) Antibody.
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