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Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551)

Western Blot - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551) - Antibodies.com Immunohistochemistry - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551) - Antibodies.com Immunofluorescence - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to PKC theta/PRKCQ (phospho Ser676) for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-PKC theta/PRKCQ (phospho Ser676) Antibody
Description
Rabbit polyclonal antibody to PKC theta/PRKCQ (phospho Ser676).
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:100-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A phospho specific peptide corresponding to residues surrounding S676 of human PRKCQ.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
78 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551) - Antibodies.comEnlarge Image

Western Blot - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551)

Western blot analysis of Jurkat, using Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551) at 1:1,000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551)

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma using Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551)

Immunofluorescence analysis of methanol-fixed HeLa cells using Anti-PKC theta/PRKCQ (phospho Ser676) Antibody (A16551).
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