Western Blot - Anti-PKC zeta (phospho Thr560) Antibody [ARC1617] (A308348)
Western blot analysis of extracts of various cell lines, using Anti-PKC zeta (phospho Thr560) Antibody [ARC1617] (A308348) at 1:1,000 dilution. Both 293T cells and C6 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3s.
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