PKD expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress human forms of FLAG-tagged PKD1, FLAG-tagged PKD2 or GST-tagged PKD3. Western blot was performed using Anti-PKD Antibody (A85245) at 0.1µg/ml (top panel), Anti-FLAG (middle panel) or Anti-GST (bottom panel).
PKD expression in HEK293 cell lysate analyzed by western blot following immunoprecipitation (IP), both using Anti-PKD Antibody (A85245) at 1µg/ml and 2µg, respectively. Cells were transfected to overexpress FLAG-tagged PKD. The heavy chain of Anti-PKD Antibody (A85245) used for the IP is detected by the western blot Anti-goat secondary antibody (indicated as IgG).
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