Rabbit polyclonal antibody to PKM2 (phospho-Ser37)
Specificity
The antibody detects endogenous level of PKM2 only when phosphorylated at serine 37.
Applications
WB, IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
60kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of in vitro kinase assays carried out with purified active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant using PKM2 (phospho-Ser37)Antibody #11456.
![Western Blot - Anti-PKM Antibody [ARC50938] (A306112) - Antibodies.com](https://cdn.antibodies.com/image/catalog/306/A306112_1.jpg?profile=product_alternative)
