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Anti-Placental Alkaline Phosphatase / PLAP Antibody [H17E2] (A280537)

This product is discontinued

Anti-Placental Alkaline Phosphatase / PLAP Antibody [H17E2] (A280537) has been discontinued and is no longer available.

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Name
Anti-Placental Alkaline Phosphatase / PLAP Antibody [H17E2]
Description
Mouse monoclonal [H17E2] antibody to Placental Alkaline Phosphatase / PLAP.
Specificity
This antibody recognises human placental alkaline phosphatase, also known as alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535 amino acid ~67 kDa GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta , in seminomas and in ovarian carcinoma. Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 has been widely used for immunolocalization of germ cell tumors and may also be used for the measurement of soluble placental alkaline phosphatase by ELISA.
Applications
Flow Cytometry, ELISA, IHC-Fr
Dilutions
Flow Cytometry: 10 µg/ml, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human
Immunogen
Placental membrane.
Host
Mouse
Clonality
Monoclonal
Clone ID
H17E2
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 recognizes human placental alkaline phosphatase, also known as alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type or Placental alkaline phosphatase 1. Placental alkaline phosphatase is a 535 amino acid ~67 kDa GPI-anchored transmembrane glycoprotein bearing a 22 amino acid signal peptide and a 29 amino acid pro-peptide region, cleaved to produce the mature form. Placental alkaline phosphatase is expressed in trophoblast cells of normal human placenta (Travers et al. 1984), in seminomas (Lange et al. 1982)and in ovarian carcinoma (Tholander et al. 1990).Mouse anti Human placental alkaline phosphatase antibody, clone H17E2 has been widely used for immunolocalization of germ cell tumors and may also be used for the measurement of soluble placental alkaline phosphatase by ELISA.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.