This is a novel monoclonal antibody that stains melanocytes and tumors derived therefrom. This antibody recognizes a different antigen to Melan A and gp100 and its epitope is not destroyed by digestion with neuraminidase (i.e. its epitope is not glycosylated). This antibody labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive. This antibody is a useful tool for the identification of melanomas and clear cell sarcomas.
Applications
IF, IHC-P
Dilutions
IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Mouse, Canine
Immunogen
Melanocyte antigen.
Host
Mouse
Clonality
Monoclonal
Clone ID
PNL2
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
160 kDa / 100 kDa / <10 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-PNL2 Antibody [PNL2] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
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