Anti-POLD1 Antibody (A14670)

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This product is discontinued

Anti-POLD1 Antibody (A14670) has been discontinued and is no longer available.

View all POLD1 Antibodies.

Specifications

Name

Anti-POLD1 Antibody

Description

Rabbit polyclonal antibody to POLD1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 808-1107 of human POLD1 (NP_002682.2).

Sequence

RYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLVANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

3'-5' exodeoxyribonuclease, DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, POLD

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-POLD1 Antibody (A14670)

Western blot analysis of extracts of various cell lines, using Anti-POLD1 Antibody (A14670) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-POLD1 Antibody (A14670)

Immunofluorescence analysis of U2OS cells using Anti-POLD1 Antibody (A14670). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-POLD1 Antibody (A14670)

Immunofluorescence analysis of A549 cells using Anti-POLD1 Antibody (A14670).

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Immunofluorescence - Anti-POLD1 Antibody (A14670)

Immunofluorescence analysis of U2OS cells using Anti-POLD1 Antibody (A14670). Green:gamma H2AX (phospho S139) used for DNA damage marker. DAPI was used to stain the cell nuclei (blue). Cells were treated by UV-A laser.