POU3F3 expression in Mouse Spinal Cord (A) and Brain (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-POU3F3 Antibody (A84814) at 1µg/ml and detected by chemiluminescence.
POU3F3 expression in Neuro-2a cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-POU3F3 Antibody (A84814) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
POU3F3 expression in Neuro-2a cells (blue) (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-POU3F3 Antibody (A84814) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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Proteins predicted to interact with POU3F3
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
