Anti-PP-T Antibody [2E11] (A304862)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-PP-T Antibody [2E11] (A304862), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus. Magnification: 40X.

Western blot analysis of human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Anti-PP-T Antibody [2E11] (A304862) at 1:500 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human A431 (15 µg). Lane 3: human HEK293 (15 µg). Lane 4: human Jurkat (15 µg). Load: 15 µg. Block: 5% Skim Milk for 1 hour at room temperature. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~58 kDa.

Immunocytochemistry/Immunofluorescence analysis of human embryonic kidney epithelial cell line (HEK293), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-PP-T Antibody [2E11] (A304862), at 1:50 for 1 hour at room temperature. The secondary antibody used was Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at room temperature. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.