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Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172)

Western Blot - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.com Immunohistochemistry - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.com Immunofluorescence - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.com Immunofluorescence - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.com Immunofluorescence - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1889] antibody to PP2A-alpha + PPP2CB for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-PP2A-alpha + PPP2CB Antibody [ARC1889]
Description
Rabbit monoclonal [ARC1889] antibody to PP2A-alpha + PPP2CB.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-309 of human PP2A alpha + beta (P62714).
Sequence
WSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1889
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
36 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Western Blot - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.comEnlarge Image

Western Blot - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172)

Western blot analysis of extracts of various cell lines, using Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172)

Immunofluorescence analysis of C6 cells using Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172)

Immunofluorescence analysis of NIH-3T3 cells using Anti-PP2A-alpha + PPP2CB Antibody [ARC1889] (A309172) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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