Anti-Prion Antibody [V21-V] (A8271)

1. Western blot analysis of prion protein in mouse brain extract (A), HEK293 cells producing recombinant human prion protein (B), and untransfected HEK 293 cells (C). 200µg of total protein loaded per well.
2. Western blot analysis of prion protein was performed by Drs. Valeriy Ostapchenko and Marco Prado, Robarts Research Institute, the University of Western Ontario, London, Ontario, Canada. Lanes A-C represent extracts (30µg of total protein per lane) of PrP-KO CF-10 cells (A), mouse hippocampus (B) and HEK293 cells transfected with mouse prion protein bearing 3F4 epitope (C). Lane D corresponds to 30ng recombinant MoPrP.