Anti-PRMT1 Antibody [ARC1066] (A306779)

Name: Anti-PRMT1 Antibody [ARC1066]
Brand: Antibodies.com
SKU: A306779
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1066] antibody to PRMT1 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PRMT1 Antibody [ARC1066]

Description

Rabbit monoclonal [ARC1066] antibody to PRMT1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRMT1 (Q99873).

Sequence

MAAAEAANCIMENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1066

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Histone-arginine N-methyltransferase PRMT1, HMT2, HRMT1L2, Interferon receptor 1-bound protein 4, IR1B4, Protein arginine N-methyltransferase 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-PRMT1 Antibody [ARC1066] (A306779)

Western blot analysis of extracts of various cell lines, using Anti-PRMT1 Antibody [ARC1066] (A306779) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Immunohistochemistry - Anti-PRMT1 Antibody [ARC1066] (A306779)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PRMT1 Antibody [ARC1066] (A306779) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PRMT1 Antibody [ARC1066] (A306779)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-PRMT1 Antibody [ARC1066] (A306779) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-PRMT1 Antibody [ARC1066] (A306779)

Immunofluorescence analysis of C6 cells using Anti-PRMT1 Antibody [ARC1066] (A306779) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).