Rabbit polyclonal antibody to Progesterone Receptor (Phospho-Ser190)
Specificity
The antibody detects endogenous level of Progesterone Receptor only when phosphorylated at serine 190.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 190 (G-L-S(p)-P-A) derived from Human Progesterone Receptor.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR
Western blot analysis of extracts from SKOV3 cells untreated (lane 1) or treated with EGF (lane 2) using Progesterone Receptor (Phospho-Ser190) Antibody #11074.
Immunofluorescence staining of methanol-fixed MCF cells using Progesterone Receptor (Phospho-Ser190) Antibody #11074.
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