Rabbit polyclonal antibody to Progesterone Receptor (Phospho-Ser190)
Specificity
The antibody detects endogenous level of Progesterone Receptor only when phosphorylated at serine 190.
Applications
WB, IHC, ICC/IF
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 190 (G-L-S(p)-P-A) derived from Human Progesterone Receptor.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR
Western blot analysis of extracts from SKOV3 cells untreated (lane 1) or treated with EGF (lane 2) using Progesterone Receptor (Phospho-Ser190) Antibody #11074.
Immunofluorescence staining of methanol-fixed MCF cells using Progesterone Receptor (Phospho-Ser190) Antibody #11074.
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