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Anti-Properdin/PFC Antibody (A14732)

Western Blot - Anti-Properdin/PFC Antibody (A14732) - Antibodies.com Immunohistochemistry - Anti-Properdin/PFC Antibody (A14732) - Antibodies.com Immunofluorescence - Anti-Properdin/PFC Antibody (A14732) - Antibodies.com Immunofluorescence - Anti-Properdin/PFC Antibody (A14732) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to Properdin/PFC for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Properdin/PFC Antibody
Description
Rabbit polyclonal antibody to Properdin/PFC.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 190-469 of human CFP (NP_001138724.1).
Sequence
CPTHGAWATWGPWTPCSASCHGGPHEPKETRSRKCSAPEPSQKPPGKPCPGLAYEQRRCTGLPPCPVAGGWGPWGPVSPCPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDGEWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQQDIRHCYSIQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGRPLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEEL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
51 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-Properdin/PFC Antibody (A14732) - Antibodies.comEnlarge Image

Western Blot - Anti-Properdin/PFC Antibody (A14732)

Western blot analysis of Human plasma, using Anti-Properdin/PFC Antibody (A14732) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-Properdin/PFC Antibody (A14732) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Properdin/PFC Antibody (A14732)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Properdin/PFC Antibody (A14732) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Properdin/PFC Antibody (A14732) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Properdin/PFC Antibody (A14732)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Properdin/PFC Antibody (A14732) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Properdin/PFC Antibody (A14732) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Properdin/PFC Antibody (A14732)

Immunofluorescence analysis of U-2 OS cells using Anti-Properdin/PFC Antibody (A14732) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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