Anti-Proteasome subunit beta type 2/PSMB2 Antibody (A14796)

Western blot analysis of extracts of various cell lines, using Anti-Proteasome subunit beta type 2/PSMB2 Antibody (A14796) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using Anti-Proteasome subunit beta type 2/PSMB2 Antibody (A14796) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Proteasome subunit beta type 2/PSMB2 Antibody (A14796) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Proteasome subunit beta type 2/PSMB2 Antibody (A14796). This Western blot was performed on the immunoprecipitate using Anti-Proteasome subunit beta type 2/PSMB2 Antibody (A14796) at a dilution of 1:1000.