Mouse monoclonal [S159-5] antibody to Protocadherin Gamma (pan).
Specificity
This antibody recognizes all Protocadherin Gamma-A, -B, and -C proteins.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:1,000, ICC/IF: 1:100
Reactivity
Human, Mouse, Rat
Immunogen
Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids)
Host
Mouse
Clonality
Monoclonal
Clone ID
S159-5
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein G purification.
Concentration
1 mg/ml
Molecular Weight
~100 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cell Membrane, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Protocadherin Gamma (pan) Antibody. (D) Composite.
Western Blot - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708)
Western blot analysis of rat brain membrane showing detection of ~100 kDa Protocadherin Gamma protein using Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) at 1:1,000 for 1 hour at room temperature. Load: 10 µg. The secondary antibody used was Goat Anti-Mouse HRP at 1:200 for 1 hour at room temperature. Predicted/Observed Size: ~100 kDa.
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Protocadherin Gamma (pan) Antibody (D) Composite.
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![Immunocytochemistry/Immunofluorescence - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_1.png?profile=product_top)
![Western Blot - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_2.png?profile=product_top)
![Immunocytochemistry/Immunofluorescence - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_3.png?profile=product_top)
![Immunocytochemistry/Immunofluorescence - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_1.png?profile=product_top_thumb)
![Western Blot - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_2.png?profile=product_top_thumb)
![Immunocytochemistry/Immunofluorescence - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_3.png?profile=product_top_thumb)
![Immunocytochemistry/Immunofluorescence - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_1.png?profile=product_image)
![Western Blot - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_2.png?profile=product_image)
![Immunocytochemistry/Immunofluorescence - Anti-Protocadherin Gamma (pan) Antibody [S159-5] (A304708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/304/A304708_3.png?profile=product_image)