This antibody recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.
Immunocytochemistry staining of HEK-293 cells cotransfected using Anti-PRR7 Antibody [TRAP3/10] (A85830), (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).
Western blotting analysis of PRR7 using Anti-PRR7 Antibody [TRAP3/10] (A85830) on rat neuron lysate, and on HeLa transfectants overexpressing human PRR7, compared with non-transfected HeLa cells (negative control). Nitrocellulose membrane was probed with 1 µg/ml of mouse anti-PRR7 followed by a secondary antibody. PRR7 was detected around 35 kDa.