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Anti-PSMB10/MECL1 Antibody (A14770)

Western Blot - Anti-PSMB10/MECL1 Antibody (A14770) - Antibodies.com Immunohistochemistry - Anti-PSMB10/MECL1 Antibody (A14770) - Antibodies.com Immunohistochemistry - Anti-PSMB10/MECL1 Antibody (A14770) - Antibodies.com Immunofluorescence - Anti-PSMB10/MECL1 Antibody (A14770) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to PSMB10/MECL1 for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-PSMB10/MECL1 Antibody
Description
Rabbit polyclonal antibody to PSMB10/MECL1.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).
Sequence
MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
29 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-PSMB10/MECL1 Antibody (A14770) - Antibodies.comEnlarge Image

Western Blot - Anti-PSMB10/MECL1 Antibody (A14770)

Western blot analysis of extracts of THP-1 cells, using Anti-PSMB10/MECL1 Antibody (A14770) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-PSMB10/MECL1 Antibody (A14770) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PSMB10/MECL1 Antibody (A14770)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-PSMB10/MECL1 Antibody (A14770) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-PSMB10/MECL1 Antibody (A14770) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PSMB10/MECL1 Antibody (A14770)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-PSMB10/MECL1 Antibody (A14770) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-PSMB10/MECL1 Antibody (A14770) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-PSMB10/MECL1 Antibody (A14770)

Immunofluorescence analysis of HeLa cells using Anti-PSMB10/MECL1 Antibody (A14770). DAPI was used to stain the cell nuclei (blue).
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