Synthetic peptide within amino acids 175-225 of human PSMD11 (NP_002806. 2).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
200 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Buffered Saline with 0.1% BSA and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Samples: Whole cell lysate from HeLa (5, 15 and 50 µg ), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Anti-PSMD11 Antibody (A297656) was used at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 3 minutes.
Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Anti-PSMD11 Antibody (A297656) was used for WB at 0.4 µg/ml and used for IP at 3 µg/mg lysate. PSMD11 was also immunoprecipitated using a rabbit anti-PSMD11 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time 10 seconds.
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