Western Blot - Anti-PSMD14 Antibody [ARC1655] (A309212)
Western blot analysis of extracts of various cell lines, using Anti-PSMD14 Antibody [ARC1655] (A309212) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-PSMD14 Antibody [ARC1655] (A309212) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human breast tissue using Anti-PSMD14 Antibody [ARC1655] (A309212) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-PSMD14 Antibody [ARC1655] (A309212) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U-2OS cells using Anti-PSMD14 Antibody [ARC1655] (A309212) at a dilution of 1:100 (40x lens). The green color is marked with alpha-Tubulin. DAPI was used to stain the cell nuclei (blue).