Recombinant chimeric monoclonal [4F2] antibody to pThemis.
Applications
ELISA
Dilutions
ELISA: 1:5,000-10,000
Reactivity
Human
Host
Rabbit / Human
Clonality
Monoclonal
Clone ID
4F2
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%-8% Trehalose is added as protectants before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
This antibody is a recombinant rabbit chimeric monoclonal antibody containing human IgG1 Fc.
Anti-pThemis Antibody [4F2] - BSA and Azide free (A324657)
ELISA examination of Anti-pThemis Antibody [4F2] - BSA and Azide free (A324657) specificity. The antibody at various concentrations was incubated in plates coated with either THEMIS or phosphorylated THEMIS (pTHEMIS), and a sigmoidal curve was developed only when using pTHEMIS, but not THEMIS, as capture antigen, indicating a specific binding of Anti-pThemis Antibody [4F2] - BSA and Azide free (A324657) to pTHEMIS.
Publishing research using Recombinant Anti-pThemis Antibody [4F2] - BSA and Azide free (A324657)? Please let us know so that we can list the citation on this page.
![ELISA - Anti-pThemis Antibody [4F2] - BSA and Azide free (A324657) - Antibodies.com](https://cdn.antibodies.com/image/catalog/324/A324657_1.jpg?profile=product_top)
![ELISA - Anti-pThemis Antibody [4F2] - BSA and Azide free (A324657) - Antibodies.com](https://cdn.antibodies.com/image/catalog/324/A324657_1.jpg?profile=product_top_thumb)
![ELISA - Anti-pThemis Antibody [4F2] - BSA and Azide free (A324657) - Antibodies.com](https://cdn.antibodies.com/image/catalog/324/A324657_1.jpg?profile=product_image)