Anti-PU.1/Spi1 Antibody (A308624)

Name: Anti-PU.1/Spi1 Antibody
Brand: Antibodies.com
SKU: A308624
Price: 270.0 GBP
Availability: InStock

Enlarge Image

$355

Rabbit polyclonal antibody to PU.1/Spi1 for WB and IHC.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PU.1/Spi1 Antibody

Description

Rabbit polyclonal antibody to PU.1/Spi1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 17-98 of human SPI1 (NP_003111.2).

Sequence

SEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

42 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

transcription factor spi1, 31 kDa Transforming Protein, 31 kDa-transforming protein, cb1086, Hematopoietic transcription factor PU.1, OF, oncogene spi1, PU.1, SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of, SFPI1, si:by184l24.2, SPI 1, SPI 1 proto oncogene, SPI A, Spi1, SPI1_HUMAN, Spleen focus forming virus (SFFV) proviral integration oncogene spi1, Spleen focus forming virus proviral integration oncogene spi1, Transcription factor PU.1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Enlarge Image

Western Blot - Anti-PU.1/Spi1 Antibody (A308624)

Western blot analysis of extracts of various cell lines, using Anti-PU.1/Spi1 Antibody (A308624) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Enlarge Image

Immunohistochemistry - Anti-PU.1/Spi1 Antibody (A308624)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-PU.1/Spi1 Antibody (A308624) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Publishing research using Anti-PU.1/Spi1 Antibody (A308624)? Please let us know so that we can list the citation on this page.