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Anti-PUF60/FIR Antibody (A15340)

Western Blot - Anti-PUF60/FIR Antibody (A15340) - Antibodies.com Western Blot - Anti-PUF60/FIR Antibody (A15340) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to PUF60/FIR for WB and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-PUF60/FIR Antibody
Description
Rabbit polyclonal antibody to PUF60/FIR.
Applications
WB, IP
Dilutions
WB: 1:500-1:2,000, IP: 1:20-1:50
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2).
Sequence
DPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
65 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-PUF60/FIR Antibody (A15340) - Antibodies.comEnlarge Image

Western Blot - Anti-PUF60/FIR Antibody (A15340)

Western blot analysis of extracts of various cell lines, using Anti-PUF60/FIR Antibody (A15340) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-PUF60/FIR Antibody (A15340) - Antibodies.comEnlarge Image

Western Blot - Anti-PUF60/FIR Antibody (A15340)

Immunoprecipitation analysis of 150µg extracts of A549 cells using 3µg of Anti-PUF60/FIR Antibody (A15340). This Western blot was performed on the immunoprecipitate using Anti-PUF60/FIR Antibody (A15340) at a dilution of 1:500.
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