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Anti-PUF60/FIR Antibody (A83473)

Western Blot - Anti-PUF60/FIR Antibody (A83473) - Antibodies.com Immunohistochemistry - Anti-PUF60/FIR Antibody (A83473) - Antibodies.com Immunohistochemistry - Anti-PUF60/FIR Antibody (A83473) - Antibodies.com
$495
Product Datasheet

Goat polyclonal antibody to PUF60/FIR for ELISA, WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-PUF60/FIR Antibody
Description
Goat polyclonal antibody to PUF60/FIR.
Applications
ELISA, WB, IHC
Dilutions
ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IHC: 5µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human PUF60/FIR.
Sequence
C-YDQERFDNSDLSA
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
60 kDa
Predicted MW
59.9kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-PUF60/FIR Antibody (A83473) - Antibodies.comEnlarge Image

Western Blot - Anti-PUF60/FIR Antibody (A83473)

PUF60/FIR expression in HEK293 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-PUF60/FIR Antibody (A83473) at 0.1µg/ml and detected by chemiluminescence.
Immunohistochemistry - Anti-PUF60/FIR Antibody (A83473) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PUF60/FIR Antibody (A83473)

PUF60/FIR expression in Human Prostate analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PUF60/FIR Antibody (A83473) at 5µg/ml and revealed with alkaline phosphatase (AP).
Immunohistochemistry - Anti-PUF60/FIR Antibody (A83473) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-PUF60/FIR Antibody (A83473)

PUF60/FIR expression in Human Skin analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-PUF60/FIR Antibody (A83473) at 5µg/ml and revealed with alkaline phosphatase (AP).
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