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Anti-PURA Antibody (A390)

Identification of endogenous Pura protein in whole cell extracts of HeLa cells and MCF-7 cells. Arrow indicates the position of PURA bands The anti-Pura antibody was used at 1/1,000 dilution. 12.5% SDS-PAGE was used. Blotting was done in wet system at 15 v overnight. CanGetSignal (Toyobo, Osaka) was used as a signal enhancer Identification of Pura protein band by western blotting, using siRNA. HCT116 cells were transfected with siPura and the cell lysates were prepared after 48 h. The Pura band was indicated by an arrow at 38 kDa position. Immunoprecipitation of Pura protein from whole cell lysate of HeLa cells with anti-PURA antibody. Whole cell lysate of HeLa cells was reacted with anti-Pura antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-Pura antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP was used. Immunofluorescence attaining of Pura protein in HeLa cells with Anti-Pura antibody. The anti-Pura antibody was used at 1/100 dilution and as the second antibody, Alexa 555-conjugated goat anti-rabbit IgG antibody was used at 1/1,000 dilution. DNA was stained with DAPI.
$555
Product Datasheet

Rabbit polyclonal antibody to PURA for WB, IP and IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-PURA Antibody
Description
Rabbit polyclonal antibody to PURA.
Applications
WB, IP, IF
Dilutions
WB: 1:1,000-1:3,000, IP: 1:1,000, IF: 1:100-1:500
Reactivity
Human, Mouse
Immunogen
Recombinant full length human PURA protein (GST tag), expressed in E. coli.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
PUR1, Purine-rich single-stranded DNA-binding protein alpha, Transcriptional activator protein Pur-alpha
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Identification of endogenous Pura protein in whole cell extracts of HeLa cells and MCF-7 cells. Arrow indicates the position of PURA bands The anti-Pura antibody was used at 1/1,000 dilution. 12.5% SDS-PAGE was used. Blotting was done in wet system at 15 v overnight. CanGetSignal (Toyobo, Osaka) was used as a signal enhancerEnlarge Image

SDS-PAGE - Anti-PURA Antibody (A390)

Anti-PURA Antibody
Identification of Pura protein band by western blotting, using siRNA. HCT116 cells were transfected with siPura and the cell lysates were prepared after 48 h. The Pura band was indicated by an arrow at 38 kDa position.Enlarge Image

Anti-PURA Antibody (A390)

Anti-PURA Antibody
Immunoprecipitation of Pura protein from whole cell lysate of HeLa cells with anti-PURA antibody. Whole cell lysate of HeLa cells was reacted with anti-Pura antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-Pura antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP was used.Enlarge Image

Immunoprecipitation - Anti-PURA Antibody (A390)

Anti-PURA Antibody
Immunofluorescence attaining of Pura protein in HeLa cells with Anti-Pura antibody. The anti-Pura antibody was used at 1/100 dilution and as the second antibody, Alexa 555-conjugated goat anti-rabbit IgG antibody was used at 1/1,000 dilution. DNA was stained with DAPI.Enlarge Image

Immunofluorescence - Anti-PURA Antibody (A390)

Anti-PURA Antibody
Publishing research using Anti-PURA Antibody (A390)? Please let us know so that we can list the citation on this page.

Proteins predicted to interact with PURA

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
98.6% Relevancy Score
94.7% Relevancy Score
89.2% Relevancy Score
88.4% Relevancy Score
87.4% Relevancy Score
82.2% Relevancy Score
82.1% Relevancy Score
81.1% Relevancy Score
80.4% Relevancy Score