Western Blot - Anti-PXN (phospho Y118) Antibody (A90529)
Western blot analysis of extracts of HeLa cells, using Anti-PXN (phospho Y118) Antibody (AP0766) at 1:2000 dilution. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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