Western blot analysis of extracts of various cell lines, using Anti-Rad50 Antibody (A11606) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using Anti-Rad50 Antibody (A11606) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Rad50 Antibody (A11606) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 150µg extracts of MCF7 cells using 3µg of Anti-Rad50 Antibody (A11606). This Western blot was performed on the immunoprecipitate using Anti-Rad50 Antibody (A11606) at a dilution of 1:500.
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