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Anti-Rad51 Antibody (A350)

SDS-PAGE of Anti-Rad51 Antibody. Western blot analysis of Rad51 in HeLa cell extract (1:2,000 dilution). Detection of Rad51 protein foci formation with Anti-Rad51 Antibody in X-ray irradiated human fibroblast cells, GM0637. 1) The cells were irradiated with X-ray at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde for 10 min and washed with PBS three times. 2) Permeabilized by treatment with 0.5% Triton for 5 min and washed with PBS three times. 3) Incubated with Anti-Rad51 Antibody at 1:100 dilution or 1:1,000 dilution for 30 min at 37°C and washed with PBS three times. 4) Anti-Chicken IgY Antibody (Alexa488) was used as a secondary at 1:5,000 dilution. Immunoprecipitation of Rad51 from crude extract of HeLa cells Rad51 was immunoprecipitated from HeLa cell extract, 20 µg, with anti-Rad51 IgY conjugated to Sepharose 4B and the eluate was immunoblotted with rabbit anti-Rad51 antibody (BioAcademia 70-012)
$530
Product Datasheet

Chicken polyclonal antibody to Rad51 for WB, IP, ChIP and IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-Rad51 Antibody
Description
Chicken polyclonal antibody to Rad51.
Applications
WB, IP, ChIP, IF
Dilutions
WB: 1:1,000-1:5,000, IP: 1:100-1:1,000, IF: 1:100-1:1,000
Reactivity
Human, Mouse, Rat, Hamster, Xenopus
Immunogen
Recombinant full length human Rad51 protein.
Host
Chicken
Clonality
Polyclonal
Isotype
IgY
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Purity
> 90% (by SDS-PAGE)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DNA repair protein RAD51 homolog 1, hRAD51, HsRAD51, RAD51 homolog A, RAD51A, RECA
Isotype Controls
Suitable Secondaries
See all Anti-Chicken IgY Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

SDS-PAGE of Anti-Rad51 Antibody.Enlarge Image

SDS-PAGE - Anti-Rad51 Antibody (A350)

Anti-Rad51 Antibody
Western blot analysis of Rad51 in HeLa cell extract (1:2,000 dilution).Enlarge Image

Anti-Rad51 Antibody (A350)

Anti-Rad51 Antibody
Detection of Rad51 protein foci formation with Anti-Rad51 Antibody in X-ray irradiated human fibroblast cells, GM0637. 1) The cells were irradiated with X-ray at 2 Gy, grown for 1 hr, fixed with 4% paraformaldehyde for 10 min and washed with PBS three times. 2) Permeabilized by treatment with 0.5% Triton for 5 min and washed with PBS three times. 3) Incubated with Anti-Rad51 Antibody at 1:100 dilution or 1:1,000 dilution for 30 min at 37°C and washed with PBS three times. 4) Anti-Chicken IgY Antibody (Alexa488) was used as a secondary at 1:5,000 dilution.Enlarge Image

Anti-Rad51 Antibody (A350)

Anti-Rad51 Antibody
Immunoprecipitation of Rad51 from crude extract of HeLa cells Rad51 was immunoprecipitated from HeLa cell extract, 20 µg, with anti-Rad51 IgY conjugated to Sepharose 4B and the eluate was immunoblotted with rabbit anti-Rad51 antibody (BioAcademia 70-012)Enlarge Image

Immunoprecipitation - Anti-Rad51 Antibody (A350)

Anti-Rad51 Antibody
Publishing research using Anti-Rad51 Antibody (A350)? Please let us know so that we can list the citation on this page.

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Detection of endogenous Rad51 in whole cell extract of HeLa cells. Anti-Rad51 Antibody was used at 1:2,000 dilution.
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Proteins predicted to interact with Rad51

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score
99.8% Relevancy Score