Western Blot - Anti-RARS Antibody [ARC65518] (A329792)
RARS expression in 293T cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-RARS Antibody [ARC65518] (A329792) at a dilution of 1:1000 and detected with chemiluminescence.
RARS expression in human colon tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-RARS Antibody [ARC65518] (A329792) at a dilution of 1:200.
Confocal imaging of paraffin-embedded Mouse brain tissue using Anti-RARS Antibody [ARC65518] (A329792) at a dilution of 1:200 following further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) at a dilution of 1:500) (red). Nuclei were stained with DAPI (blue). Antigen retrieval was achieved by microwaving in 0.01M citrate buffer, pH 6.0. Objective: 40x.
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