Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Calcium and DAG-regulated guanine nucleotide exchange factor III, GRP3, Guanine nucleotide exchange factor for Rap1, KIAA0846, Ras guanyl-releasing protein 3
Western blot analysis of extracts of various cell lines, using Anti-RASGRP3 Antibody (A12171) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
