Use 10µl of the suggested working dilution to label 106 cells in 100µl
Immunogen
Horseradish peroxidase.
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat IgG1 negative control, a rat monoclonal raised against horseradish peroxidase, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG1 test antibodies to the surface of human and mouse cells in flow cytometry.Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow Cytometry - Rat IgG1 (PE) - Isotype Control (A282639)
Figure A. A488 conjugated Hamster anti Mouse CD3 and Rat IgG1 (PE) - Isotype Control (A282639). Figure B. A488 conjugated Hamster anti Mouse CD3 and Anti-CD22 Antibody [OX-97] (PE) (A281521). Alll experiments performed on mouse splenocytes in the presence of murine Seroblock.
Flow Cytometry - Rat IgG1 (PE) - Isotype Control (A282639)
Figure A. PE-A750 tandem-conjugated Rat anti Mouse CD45R and Rat IgG1 (PE) - Isotype Control (A282639). Figure B. PE-A750 tandem-conjugated Rat anti Mouse CD45R and Anti-SLAM / CD150 Antibody [9D1] (PE) (A279567). All experiments performed on mouse splenocytes in the presence of murine SeroBlock.
Flow Cytometry - Rat IgG1 (PE) - Isotype Control (A282639)
Figure A. Anti-CD3 Antibody [CA17.2A12] (FITC) (A279975) and Rat IgG1 (PE) - Isotype Control (A282639). Figure B. Anti-CD3 Antibody [CA17.2A12] (FITC) (A279975) and Anti-CD8 Antibody [YCATE55.9] (PE) (A282026). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
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