Use 10µl of the suggested working dilution to label 106 cells in 100µl
Immunogen
Human lymphocytes.
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat IgG2a Negative Control antibody is suitable for the assessment of the level of non-specific binding of rat IgG2a monoclonal antibodies to mouse cells. Test results indicate Rat IgG2a Negative Control antibody is also suitable for use as a negative control with canine cells. N.B. This antibody recognizes a human cell surface marker, and therefore is not suitable as a negative control in human cells or cell lines.
Flow Cytometry - Rat IgG2a (PE) - Isotype Control (A282637)
Figure A. A647 conjugated Rat anti Mouse CD45R and Rat IgG2a (PE) - Isotype Control (A282637). Figure B. A647 conjugated Rat anti Mouse CD45R and Anti-CD40 Antibody [3/23] (PE) (A278860). All experiments performed on mouse splenocytes in the presence of mouse SeroBlock
Flow Cytometry - Rat IgG2a (PE) - Isotype Control (A282637)
Figure A. Anti-CD45R Antibody [RA3-6B2] (FITC) (A281643) and Rat IgG2a (PE) - Isotype Control (A282637). Figure B. Anti-CD45R Antibody [RA3-6B2] (FITC) (A281643) and Anti-CD48 Antibody [OX-78] (PE) (A281494). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock
Flow Cytometry - Rat IgG2a (PE) - Isotype Control (A282637)
Figure A. Anti-CD3 Antibody [CA17.2A12] (FITC) (A279975) and Rat IgG2a (PE) - Isotype Control (A282637). Figure B. Anti-CD3 Antibody [CA17.2A12] (FITC) (A279975) and Anti-CD4 Antibody [YKIX302.9] (PE) (A282042). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
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