The antibody detects endogenous level of RB only when phosphorylated at serine 811.
Applications
WB, IF
Dilutions
Western blotting: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200
Reactivity
Human, Mouse, Rat
Immunogen
A phospho specific peptide corresponding to residues surrounding S811 of human RB.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C
Synonyms
p105-Rb, p110-RB1, pp110, pRb, RB1, Retinoblastoma-associated protein