Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Histone acetyltransferase type B subunit 2, Histone-binding protein RBBP7, Nucleosome-remodeling factor subunit RBAP46, RBBP-7, Retinoblastoma-binding protein 7, Retinoblastoma-binding protein p46
Western blot analysis of extracts of various cell lines, using Anti-RBBP7 Antibody (A90109) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western blot analysis of extracts of Rat spleen, using Anti-RBBP7 Antibody (A90109) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Chromatin immunoprecipitation (ChIP) analysis of extracts of 293T cells, using Anti-RBBP7 Antibody (A90109) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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