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Anti-REDD-1/DDIT4 Antibody (A10292)

Western Blot - Anti-REDD-1/DDIT4 Antibody (A10292) - Antibodies.com Western Blot - Anti-REDD-1/DDIT4 Antibody (A10292) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to REDD-1/DDIT4 for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-REDD-1/DDIT4 Antibody
Description
Rabbit polyclonal antibody to REDD-1/DDIT4.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-232 of human DDIT4 (NP_061931.1).
Sequence
MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
32 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-REDD-1/DDIT4 Antibody (A10292) - Antibodies.comEnlarge Image

Western Blot - Anti-REDD-1/DDIT4 Antibody (A10292)

Western blot analysis of extracts from normal (control) and DDIT4 knockout (KO) HeLa cells, using Anti-REDD-1/DDIT4 Antibody (A10292) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Western Blot - Anti-REDD-1/DDIT4 Antibody (A10292) - Antibodies.comEnlarge Image

Western Blot - Anti-REDD-1/DDIT4 Antibody (A10292)

Western blot analysis of extracts of various cell lines, using Anti-REDD-1/DDIT4 Antibody (A10292) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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