Anti-REN Antibody (A101593)

Western blot analysis of lysates from Jurkat cells using Anti-REN Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Protein expression of key renin-angiotensin system (RAS) components in MCF-7 breast cancer cells under different treatment conditions. MCF-7 cells were treated for 48 h with liquorice root extract (LRE; 0.1 mg/mL), conditioned medium from preadipocytes (preAdip-CM), mature adipocytes (matAdip-CM), or LRE-treated mature adipocytes (matAdip + LRE-CM), and compared with untreated control cells (CTL). Protein expression of angiotensinogen and renin was assessed by Western blot using Anti-Angiotensinogen Antibody (A90383) and Anti-REN Antibody (A101593) (Antibodies.com), respectively, with GAPDH used as a loading control. Bar graphs show densitometric quantification of protein expression levels. Values are presented as mean ± SD from three independent experiments (n = 3). Different letters indicate statistically significant differences (p < 0.05).