Rabbit polyclonal antibody to RFA2 (Phospho-Thr21)
Specificity
The antibody detects endogenous level of RFA2 only when phosphorylated at Threonine 21.
Applications
WB
Reactivity
Human, Rat
Immunogen
Peptide sequence around phosphorylation site of Threonine 21 (G-Y-T(p)-Q-S) derived from Human RFA2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Rat heart using RFA2 (Phospho-Thr21) Antibody #12060.The lane on the right is treated with the antigen-specific peptide.