Anti-RFC4 Antibody (A14797)

Name: Anti-RFC4 Antibody
Brand: Antibodies.com
SKU: A14797
Price: 280.0 GBP
Availability: InStock

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This product is discontinued

Anti-RFC4 Antibody (A14797) has been discontinued and is no longer available.

View all RFC4 Antibodies.

Specifications

Name

Anti-RFC4 Antibody

Description

Rabbit polyclonal antibody to RFC4.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:100, IP: 1:50-1:200

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 204-363 of human RFC4 (NP_002907.1).

Sequence

DKIQQQRLLDIAKKENVKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C 37 kDa subunit, Replication factor C subunit 4, RF-C 37 kDa subunit, RFC37

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-RFC4 Antibody (A14797)

Western blot analysis of extracts of various cell lines, using Anti-RFC4 Antibody (A14797) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunofluorescence - Anti-RFC4 Antibody (A14797)

Immunofluorescence analysis of U2OS cells using Anti-RFC4 Antibody (A14797). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-RFC4 Antibody (A14797)

Immunofluorescence analysis of U2OS cells using Anti-RFC4 Antibody (A14797). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-RFC4 Antibody (A14797)

Immunoprecipitation analysis of 200µg extracts of K562 cells using 1µg of Anti-RFC4 Antibody (A14797). This Western blot was performed on the immunoprecipitate using Anti-RFC4 Antibody (A14797) at a dilution of 1:1000.