Anti-Rhodopsin Antibody [4D2] (A305179)

Western blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Anti-Rhodopsin Antibody [4D2] (A305179) at 1:1,000. Lane 1: MW ladder. Lane 2: 10 ug. Lane 3: 5 ug. Lane 4: 2.5 ug..

Western blot analysis of human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Anti-Rhodopsin Antibody [4D2] (A305179) at 1:1,000 for 2. Lane 1: MW ladder. Lane 2: human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. 5 hours at room temperature with shaking. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:1,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.

Immunohistochemistry analysis of mouse retina. The Primary Antibody used was Anti-Rhodopsin Antibody [4D2] (A305179) at 1:1,000. The secondary antibody used was FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..