Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
REPA1, Replication factor A protein 1, Replication protein A 70 kDa DNA-binding subunit, RF-A protein 1, RP-A p70, RPA1, Single-stranded DNA-binding protein
Western Blot - Anti-RPA70 Antibody [ARC0773] (A307959)
Western blot analysis of extracts of various cell lines, using Anti-RPA70 Antibody (A307959) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-RPA70 Antibody [ARC0773] (A307959)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-RPA70 Antibody (A307959). This Western blot was performed on the immunoprecipitate using Anti-RPA70 Antibody (A307959) at a dilution of 1:1000.
Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-RPA70 Antibody (A307959) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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