This antibody recognises DNA-directed RNA polymerase II subunit RPB2.RPB2 is the second largest subunit of RNA polymerase II, the polymerase responsible for the synthesis of messenger RNA in eukaryotic cells. The RPB2 subunit combines with other polymerase subunits to form a structure within RNA polymerase II that maintains the contact between the DNA and the newly synthesized RNA within the active site of the enzyme.
Applications
IP, WB
Dilutions
IP: 5 µl per IP - 10 µl per IP, WB: 1:500 - 1:1,000
Reactivity
Human
Immunogen
A peptide derived from within the N-terminus of human RPB2.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Human RPB2 antibody recognizes DNA-directed RNA polymerase II subunit RPB2.RPB2 is the second largest subunit of RNA polymerase II, the polymerase responsible for the synthesis of messenger RNA in eukaryotic cells. The RPB2 subunit combines with other polymerase subunits to form a structure within RNA polymerase II that maintains the contact between the DNA and the newly synthesized RNA within the active site of the enzyme (Acker et al. 1997).
