Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
60S ribosomal protein L10a, CSA-19, Large ribosomal subunit protein uL1, NEDD-6, NEDD6, Neural precursor cell expressed developmentally down-regulated protein 6
Western blot analysis of extracts of various cell lines, using Anti-RPL10A Antibody (A15055) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded rat cerebellum using Anti-RPL10A Antibody (A15055) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-RPL10A Antibody (A15055) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Anti-RPL10A Antibody (A15055) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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Proteins predicted to interact with RPL10A
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.